[Retracted] Insight into the Protective Effect of Salidroside against H₂O₂-Induced Injury in H9C2 Cells

Figure 4

Salidroside elevated the protein expression of Bcl-2 and inhibited cell apoptosis in H₂O₂-treated H9C2 cells. (a) H9C2 cells were transfected with siRNA targeting Bcl-2 (siBcl-2) or negative control (NC) using Lipofectamine3000 for 48 h and then harvested. H9C2 cells were transfected with siBcl-2 or NC for 24 h, and then the cells were treated with or without salidroside (Sal, 100 μM) for 24 h and then stimulated with or without H₂O₂ (400 μM) for another 4 h. (b) The protein expressions were detected by western blotting. (c) The morphological changes of apoptotic cells were observed by Hochest staining; (d) The apoptotic H9C2 cells were detected by flow cytometry. Experiments have been repeated four times. The values represent as . .

(a)

(b)

(c)

(d)