Folic Acid Protects Melanocytes from Oxidative Stress via Activation of Nrf2 and Inhibition of HMGB1

<div>FA inhibited the production of HMGB1 in PIG1 cells treated with H<sub>2</sub>O<sub>2</sub>. (a) The mRNA level of HMGB1 normalized to <i>β</i>-actin in PIG1 cells treated with H<sub>2</sub>O<sub>2</sub> and FA at indicated concentrations for 24 h was determined via qRT-PCR. (b) The protein level of HMGB1 in PIG1 cells treated with H<sub>2</sub>O<sub>2</sub> and FA at indicated concentrations for 48 h was determined via Western blot. <i>β</i>-Actin was detected as loading control. (c) The secretion level of HMGB1 from PIG1 cells treated with H<sub>2</sub>O<sub>2</sub> and FA at indicated concentrations for 72 h was determined via ELISA. 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Folic Acid Protects Melanocytes from Oxidative Stress via Activation of Nrf2 and Inhibition of HMGB1 