Jie-Yu-He-Huan Capsule Ameliorates Anxiety-Like Behaviours in Rats Exposed to Chronic Restraint Stress via the cAMP/PKA/CREB/BDNF Signalling Pathway

Figure 8

Treatment with Jie-Yu-He-Huan capsule prevents alterations of cyclic adenosine monophosphate response element-binding protein (CREB), phosphorylated- (p-) CREB, and brain-derived neurotrophic factor (BDNF) caused by chronic restraint stress. (a) CREB mRNA expression in the prefrontal cortex (PFC). (b) CREB protein expression in the PFC. (c) Phosphorylated- (p-) CREB protein expression in the PFC. (d) CREB mRNA expression in the hippocampus (Hippo). (e) CREB protein expression in the Hippo. (f) p-CREB protein expression in the Hippo. (g) BDNF mRNA expression in the PFC. (h) BDNF protein expression in the PFC. (i) BDNF mRNA expression in the Hippo. (j) BDNF protein expression in the Hippo. (k) Representative western blot protein bands of CREB, p-CREB, and BDNF expression showing differences among groups. compared to the control group, compared to the control group, compared to the control group, ^# compared to the model group, and ^## compared to the model group ( in each group, unpaired -test). Data are expressed as the . The small circles represent individual data points.

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