Berberine Suppresses EMT in Liver and Gastric Carcinoma Cells through Combination with TGFβR Regulating TGF-β/Smad Pathway
Effects of BBR on epithelial-mesenchymal transition (EMT) in HCC cells and gastric carcinoma cells. (a) HepG2, SGC7901, and MGC803 cells were treated with BBR (10, 20, and 40 μM) for 24 h. The level of EMT markers, including E-cadherin, ZO-1, N-cadherin, vimentin, Snail, and Slug, was assessed by Western blotting assays. (b) When treated with BBR (10, 20, and 40 μM), vimentin was determined by confocal microscopy in HepG2, SGC7901, and MGC803 cells, Vimentin-positive expression was indicated by green fluorescence, and blue fluorescence indicates 4,6-diamidino-2-phenylindole- (DAPI-) labeled nuclei. Scale bars: 20 μm. Representative images and typical graphs () are shown, (., versus the control group).
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