Chronic Intermittent Hypoxia Regulates CaMKII-Dependent MAPK Signaling to Promote the Initiation of Abdominal Aortic Aneurysm

Figure 6

CIH damages VSMCs through CaMKII-dependent MAPK signaling. The isolated VSMCs from mouse aortas tissues were stimulated with Ang II combined with CIH. An inhibitor of calcium channel (CaMKII-IN-1) was used to treat VSMCs. (a) Representative image of Western blot. (b) Quantification of CaMKII, p38, and Jnk from Western blot (). (c) Representative image of Western blot. (d) Quantification of MMP2, MMP9, and MCP1 from Western blot (). (e) Representative images showing mtROS levels (). (f) Representative image of flow cytometry for testing apoptosis. (g) Quantification of apoptotic cells from flow cytometry (). (h) Representative image of Western blot. (i) Quantification of Bax, Bcl-2, and cleaved caspase-3 expressions (). , .

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