Research Article

MicroRNA-145-Mediated KDM6A Downregulation Enhances Neural Repair after Spinal Cord Injury via the NOTCH2/Abcb1a Axis

Figure 5

miR-145 downregulates Abcb1a by inhibiting NOTCH2 expression through KDM6A. (a) qRT-PCR to measure the relative expression of KDM6A mRNA in the spinal cord tissue of each group of rats (). (b) Western blot assay to measure the expression of KDM6A, NOTCH2, and Abcb1a proteins in LPS-induced cells. (c) ChIP assay with KDM6A antibody. (d) ChIP assay with H3K27me3 antibody. (e) Western blot assay to measure the protein levels of H3K27me3, NOTCH2, and Abcb1a after treatment with GSK-J. Data were expressed as . Independent sample -test was employed for data comparison between two groups. Data comparison among multiple groups was performed using one-way ANOVA with Tukey’s post hoc test. versus the sham-operated rats, mimic-NC+oe-NC group, miR-145-mimic+oe-NC group, or mimic-NC+LPS group. # versus the SCI+miR-145 group, mimic-NC+oe-NC+LPS group, miR-145-mimic+LPS group, or IgG group (, , ). Cell experiments were repeated three times.
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