Pharmacological Activation of Nrf2 by Rosolic Acid Attenuates Endoplasmic Reticulum Stress in Endothelial Cells

Figure 7

Analysis of apoptotic frequencies. Rosolic acid-treated EA.hy926 cells under ER stress was measured by flow cytometry using the Annexin-V labelling assay. Cells were pretreated with 5 and 10 μg for 24 hours and then treated with Thapsigargin for 2 hours and then incubated with FITC-labelled Annexin-V. After washing, the cells were subjected to flow cytometric analysis. The intensity of the Annexin-V-Fluos signal is represented on the -axis. A significant peak shift towards right was observed in the endothelial cells exposed to TPG (c) in comparison with the untreated cells (a) whereas a dose-dependent shift was observed towards the control level in the RA-pretreated endothelial cells (d, e). No peak shift was observed in the cells exposed only RA (b). The apoptotic population (%) against the effect of Rosolic acid against the TPG-induced ER stress in EA.hy926 cells (f).

(a)

(b)

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(e)

(f)