Pharmacological Activation of Nrf2 by Rosolic Acid Attenuates Endoplasmic Reticulum Stress in Endothelial Cells

Figure 9

Effect of Rosolic acid on ER stress-induced toxicity in Nrf2 KO and wild-type EA.hy926 cells. (a) Cytoprotective effect of Rosolic acid in wild-type and Nrf2 KO EA.hy926 cells against thapsigargin-induced toxicity as assessed by MTT assay. (b) Effect of Rosolic acid on ER stress-induced toxicity in Nrf2 KO and wild-type cells as assessed by ER stress markers, (b) protein kinase R- (PKR-) like endoplasmic reticulum kinase (PERK), (c) activating transcription factor- (ATF-) 6, (d) glucose-regulated protein (GRP) 78, and (e) C/ERB homologous protein (CHOP) gene expression levels in ER stress-induced in wild-type and Nrf2 KO EA.hy926 cells using thapsigargin by qRT-PCR analysis. Data are represented as of three independent experiments. Significant compared with the control group. ^#Significant compared with TPG group; determined by one-way ANOVA followed by Tukey’s post hoc test.

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