Calorie Restriction Protects against Contrast-Induced Nephropathy via SIRT1/GPX4 Activation

Figure 5

Role of SIRT1 in the antioxidants and antiferroptosis effects of CR on CIN. SD rats were pretreated with CR for 4 weeks. CIN was induced by the intravenous injection of the CM iopromide (1.8 g/kg). The SIRT1 inhibitor EX527 (500 mg/kg) was injected intravenously before establishment of CIN. The kidney samples were collected at 24 h after CM injection. (a1) Tissue oxidative stress level in the renal outer medulla, measured using DHE ( μm). (a2) The quantitative mean fluorescence intensity in each treatment group is shown. The values are presented with fold change of the control group. The renal levels of MDA (b), SOD (c), and GSH/GSSG ratio (d) were measured to determine the oxidative stress. (e) Western blots and relative band densities of GPX4 (regulator of ferroptosis) in the renal outer medulla of different groups. The values are presented as fold change of the control group. The values are presented as (, vs. control, ^# vs. CM alone, and ^& vs. CM+CR).

(a)

(b)

(c)

(d)

(e)