Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction
Activation of the tissue renin-angiotensin system (tRAS) in the human degenerated intervertebral disc tissue. (a) The volcano plot of the gene relative expression of NP tissue in the none-degenerated group compared to the severely degenerated group. Pink circles represented upexpressed proteins (), whereas black circles suggested proteins without expression differences between these two groups (). (b) The clustering analysis heat map. (c) Top 20 enriched GO terms. (d) Top ten enriched KEGG pathways. (e) TUNEL assay of the human NP tissue among grades II, III, and IV (). (f) Quantitative results of TUNEL-positive cells. (g) Immunohistochemical analysis of the expression of ACE in the human NP tissue among grades II, III, and IV (). (h) Quantitative results of ACE-positive cells. (i, j) Western blot results of the protein expression of tRAS components (ACE and AT1) and MMP13 in the human NP tissue ()..,,,.
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