Tissue Renin-Angiotensin System (tRAS) Induce Intervertebral Disc Degeneration by Activating Oxidative Stress and Inflammatory Reaction
Angiotensin II decreased cell viability and induced apoptosis in human NP cells. (a) The effects of Ang II with different concentrations on human NP cells (). (b) The IC50 curve of Ang II for human NP cells. (c) Apoptotic human NP cells were presented using the TUNEL assay ().. (d) TUNEL-positive cells were quantified (). (e) The effect of Ang II on the mitochondrial membrane potential of NP cells was detected by JC-1 staining (). (f) The ratio of JC-1 aggregates (red) to monomers (green) was quantified ().. (g) The expression of apoptosis-related proteins was analyzed by western blot (). (h) The expression of apoptosis-related proteins was quantified ().,,,.
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