TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization

Figure 3

Myeloid-specific TL1A overexpression induces macrophages polarization to M1 phenotype. (a1) Relative mRNA levels of iNOS in mice of HFHF model were determined by quantitative real-time PCR. (a2) Relative mRNA levels of CD206 in mice of HFHF model were determined by quantitative real-time PCR. (b1, b3) iNOS protein of liver macrophages was detected by immunofluorescence double staining in mice of HFHF model (400x). (b2, b4) CD206 protein of liver macrophages was detected by immunofluorescence double staining in mice of HFHF model (400x). The expression of TNF-α, IL-1β, and IL-6 in serum (c1–c3), liver tissues (d1–d3), and liver macrophages (e1–e4) of mice fed with HFHF diets was detected by ELISA, quantitative real-time PCR, and flow cytometry, respectively. Data are expressed as , ^n.s., , , .

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