Oxidative Medicine and Cellular Longevity / 2021 / Article / Fig 6

Research Article

Clostridium butyricum Protects IPEC-J2 Cells from ETEC K88-Induced Oxidative Damage by Activating the Nrf2/ARE Signaling Pathway

Figure 6

Role of Nrf2 on ETEC K88-induced damage promoted by CB. (a) IPEC-J2 cells incubated with Nrf2-specific siRNA (siRNA) and Nrf2 nonspecific siRNA control (NC) for 6 h. After pretreatment with CB for 3 h, the cells were treated with  cfu/mL ETEC K88 for 3 h, 6 h, 12 h, and 24 h. The contents of Nrf2 were determined by qPCR. (b) After pretreatment with CB for 3 h, the cells were treated with 103 cfu/mL ETEC K88 for 12 h. Determination of Nrf2 proteins content by Western blot. The results were of three independent preparations. Value columns with different lowercase letters mean , while with different capital letters mean .
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