Doxycycline Hyclate Modulates Antioxidant Defenses, Matrix Metalloproteinases, and COX-2 Activity Accelerating Skin Wound Healing by Secondary Intention in Rats

Figure 3

Effects of doxycycline hyclate (Dx) on cyclooxygenase-2 (COX-2), prostaglandin E2 production, and metalloproteinases (MMP) and N-acetyl-β-D-glucosaminidase (NAG) activity in LPS-stimulated RAW264.7 macrophages treated with various doses of Dx for 24 h. NC: not stimulated cells (not treated with LPS or Dx), CM: cells treated with culture medium containing LPS, and Dx: cells treated with culture medium containing LPS and Dx at 10, 30, 100, and 300 μM. The data are expressed as mean and standard deviation. Statistical difference (Student-Newman-Keuls test, ), compared to NC, ^&CM, Dx10, and Dx30, ^#NC, CM, and Dx (10, 30, and 100), and + CM and Dx10.