The Synergistic Reducing Drug Resistance Effect of Cisplatin and Ursolic Acid on Osteosarcoma through a Multistep Mechanism Involving Ferritinophagy

Figure 2

Combination treatment with UA and Cis inhibited OS cell proliferation by increasing DNA damage in OS cells. The proliferation of OS cells was assessed by the EdU incorporation assay after monotherapy or treatment with the combination of UA and Cis for 24 h (a, b) (, scale bar: 100 μm). OS cells were treated with the combination of UA and Cis or either drug alone for 24 h, and cell cycle distribution was analysed by flow cytometry (c, d) (). OS cells were treated with the combination of UA and Cis or either drug alone for 24 h and stained with a γ-H2AX antibody (e, f) (, scale bar: 25 μm). The data are presented as the . vs. the control group.

(a)

(b)

(c)

(d)

(e)

(f)