Research Article

Protective Effects of Topical Application of Nitrite on Testicular Ischemia-Reperfusion Injury in Rats

Figure 2

Effects of topical application of nitrite and nitrate on malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and cyclic guanosine monophosphate (cGMP) levels in the ipsilateral and contralateral testes. Color black, left testis; color gray, right testis. (a) MDA values are expressed as micromoles of MDA per milligram of protein (μmol/mg protein). Data are . vs. group A left testis, # vs. group B left testis. (b, c) SOD values are expressed as unit of SOD per milligram of protein (U/mg protein), and CAT values are expressed as nanomolar/minute/milligram protein. Data are . vs. group B left testis. (d) cGMP values are expressed as femtomoles of cGMP per milligram of protein (fmol/mg protein). Data are . vs. group A left testis, # vs. group F left testis, and & vs. group C right testis. Groups: A: control, B: ischemia/reperfusion (IR) injury, C: IR+0.12 nmol/g nitrite, D: IR+1.2 nmol/g nitrite, E: IR+12 nmol/g nitrite, F: IR+0.12 nmol/g nitrite+0.01 μmol/g C-PTIO, and G: IR+0.12 nmol/g nitrate.
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