Role of SIRT1 in Hepatic Encephalopathy: In Vivo and In Vitro Studies Focusing on the NLRP3 Inflammasome

Figure 7

SIRT1 knockdown enhanced the activation of NLRP3 inflammasome. (a) The SIRT1 knockdown lentiviral vector (LV) was transfected into BV2 microglial cells. The expression of EGFP was observed by fluorescence microscope; (b) the mRNA of SIRT1 of SIRT1 knockdown cells was determined by RT–PCR; (c) the protein expression of SIRT1 of SIRT1 knockdown cells was determined by western blotting; (d) the quantitative blots of SIRT1; (e) cells were divided into four groups: control, control+10 μg/ml LPS+4 mM ATP, LV-NC+10 μg/ml LPS+4 mM ATP, and LV-down+10 μg/ml LPS+4 mM ATP group. The protein expression of SIRT1, NLRP3, ASC, and IL-1β was detected by western blotting; (f) the quantitative blots of SIRT1; (g) the quantitative blots of NLRP3, ASC, and IL-1β; (h) the gene expression of SIRT1; (i) the gene expression of IL-1β. , compared with the control group. ^#, compared with the LV-NC+10 μg/ml LPS+4 mM ATP group. per group. The data represent the .

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