Role of SIRT1 in Hepatic Encephalopathy: In Vivo and In Vitro Studies Focusing on the NLRP3 Inflammasome

Figure 9

SIRT1 knockdown enhanced the activation of NLRP3 inflammasome by regulating ROS. Cells were divided into four groups: LV-NC, LV-NC+10 μg/ml LPS+4 mM ATP, LV-down+10 μg/ml LPS+4 mM ATP, and LV-down+10 μg/ml LPS+4 mM ATP+NAC (5 mM); (a) the protein expression of SIRT1, NLRP3, ASC, and IL-1β was detected by western blotting; (b) the quantitative blots of SIRT1; (c) the quantitative blots of NLRP3, ASC, and IL-1β; (d) the gene expression of SIRT1; (e) the gene expression of IL-1β. versus LV-NC. ^# versus LV-NC+10 μg/ml LPS+4 mM ATP. ^& versus LV-down+10 μg/ml LPS+4 mM ATP. per group. The data represent the .

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