Supplemental N-3 Polyunsaturated Fatty Acids Limit A1-Specific Astrocyte Polarization via Attenuating Mitochondrial Dysfunction in Ischemic Stroke in Mice

Figure 3

DHA protects against mitochondrial dysfunction in the astrocytes under hypoxia condition. Primary astrocytes were treated with DHA for indicated time periods under hypoxia condition. (a, b) The mitochondrial membrane potential in astrocytes was measured by the membrane-permeant JC-1 staining for confocal scanning (a) and flow cytometry analysis (b). (c, d) Mitochondrial ROS was measured by MitoSOX probes (c), and the MFI of MitoSOX (d) was calculated by Image J software. (e, f) MitoTracker was employed to mark mitochondria (e). Statistical analysis was performed on 200 cells in each group to obtain five data sets; quantification of mito-tracker fluorescence intensity, number of individuals, number of networks, and number of fragmented mitochondria were analyzed using the Image-Pro Plus with MiNA on mitochondrion-labelled images (f). . . One of the three independent experiments is shown.

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