Prunus mume Seed Exhibits Inhibitory Effect on Skin Senescence via SIRT1 and MMP-1 Regulation

Figure 3

Phenotypic comparison of the mice skin surfaces with or without ultraviolet (UV) irradiation. The back skin of the BALB/c mice was photographed after UV irradiation, and 50 μl of the sample (25 mg/ml) was applied 20 times. Raw photographs were taken using a digital camera with a color marker (a). The photographs from (a) were processed to invert the red color so that the red color on the manufactured color marker and the damaged skin area were converted and shown as a bright color (b). To calculate damage to the skin, the ripped skin was transformed from a binary color image into a black and white image (c). Redness was calculated using ImageJ software according to a histogram of RGB values (d). Back skin thickness was directly calculated using a digimatic thickness gage (e). Collagen content was determined from skin lysates using a Sircol Collagen Assay Kit (f). Results are shown as the . ^# compared with the UV-nonirradiated group. compared with the UV-irradiated group. PmUF: Prunus mume unripe fruit; PmRF: P. mume ripe fruit; PmRS: P. mume ripe seed.