Bmal1 Regulates the Redox Rhythm of HSPB1, and Homooxidized HSPB1 Attenuates the Oxidative Stress Injury of Cardiomyocytes

Figure 2

The expression of homooxidized HSPB1 was decreased in the plasma of AMI patients. (a) ELISA assay quantified the plasma of HSPB1, and the absorbance was measured using a microplate spectrophotometer set to 450 nm with wavelength correction set to 630 nm. for the Am subgroup, for the Pm subgroup, and for the Eve subgroup. Error bars demonstrated of 3 replications. , . (b) The patient’s plasma was diluted 5 times with sterilized water containing 100 mM NEM alkylating agent, and then, NEM-alkylated redox Western blot analysis was performed to detect the redox state of HSPB1. For reducing SDS-PAGE, 5% dithiothreitol was added to the samples. (c) Redox potentials were determined using band intensities and the Nernst equation. for each group; blue, red, and black numbers on behalf of Am subgroup, Pm subgroup, and Eve subgroup, respectively. and .

(a)

(b)

(c)