NOX4-Derived ROS Mediates TGF-β1-Induced Metabolic Reprogramming during Epithelial-Mesenchymal Transition through the PI3K/AKT/HIF-1α Pathway in Glioblastoma

Figure 1

TGF-β1 and NOX4 are highly expressed in glioblastoma and associated with the grade of glioma. (a) Analysis of TGF-β1 and NOX4 expression in normal brain and glioma tissues based on TCGA database. (b) qPCR analysis of the TGF-β1 and NOX4 mRNA levels in the normal human brain samples and GBM tissues. (c) Western blot analysis of TGF-β1 and NOX4 protein expression in the normal human brain samples and glioma tissues. (d) Immunohistochemical staining for TGF-β1 and NOX4 protein expression in the normal human brain samples and glioma tissues.  μm. (e, f) The prognostic value of TGF-β1 and NOX4 expression in the LGGs and GBMs was analyzed with TCGA database. Data represent mean and SD of three independent experiments. ns: not significant; ; ; .

(a)

(b)

(c)

(d)

(e)

(f)