PPARγ Attenuates Interleukin-1β-Induced Cell Apoptosis by Inhibiting NOX2/ROS/p38MAPK Activation in Osteoarthritis Chondrocytes

Figure 4

Detection of oxidative stress and inflammatory factors. Cultured chondrocytes were pretreated with 10 mM NAC, 10 μM GW1929, 10 μM DPI, 10 μM SB203580, 10 μM PD98059, or 10 μM SP600125 for 1 h and then treated with 10 ng/ml IL-1β for 24 h. (a) ROS level was detected by flow cytometry, (b) PGE₂ content was detected by ELISA, and (c) COX-2 expression was detected by qRT-PCR. Results are presented as of three independent experiments. Chondrocytes cultured in DMEM were used as the vehicle control. , , and versus the vehicle control. ^#, ^##, and ^### versus the IL-1β group. ^& and ^&& versus the NAC group.

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