PPARγ Attenuates Interleukin-1β-Induced Cell Apoptosis by Inhibiting NOX2/ROS/p38MAPK Activation in Osteoarthritis Chondrocytes

Figure 6

Western blot. Cultured chondrocytes were pretreated with 10 mM NAC, 10 μM GW1929, or 10 μM DPI for 1 h and then treated with 10 ng/ml IL-1β for 24 h; western blot was used to detect the expression of PPARγ, NOX2, p38, p-p38, ERK1/2, p-ERK1/2, JNK1/2, and p-JNK1/2. Representative western blot (a) and quantification data (b–i) are shown, respectively. The relative protein levels were normalized to the level of the internal control, GAPDH, and presented as fold changes relative to the control group (the level of the control group was set as 1). Results are presented as the of three independent experiments. Chondrocytes cultured in DMEM were used as the vehicle control. , , and versus the vehicle control. ^#, ^##, and ^### versus the IL-1β group. ^& and ^&& versus the NAC group.

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