Research Article
Exploring the Role and Mechanism of pAMPKα-Mediated Dysregulation of Brf1 and RNA Pol III Genes
Figure 5
MNNG induces Brf1 expression and Pol III gene transcription. A549 cells were treated with different doses of the carcinogen MNNG. The resultant cell lysis and total RNA were extracted from the cells for immunoblotting analysis and RT-qPCR. (a) Immunoblotting analysis of cellular levels of Brf1 and pAMPKα. (b–e) RT-qPCR. Brf1 mRNA (b) and transcription levels of tRNALeu (c), 5S rRNA (d), and tRNATyr (e). The results indicate that MNNG activated pAMPKα and enhanced Brf1 expression and Pol III gene transcription. All error bars represent the SD of at least three independent experiments. values were determined by a two-tailed -test. , .
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