Ulinastatin Ameliorates IL-1β-Induced Cell Dysfunction in Human Nucleus Pulposus Cells via Nrf2/NF-κB Pathway

Figure 5

Effect of UTI on IL-1β-induced oxidative stress in human NP cells. (a) The distribution of ROS (green) was detected by the immunofluorescence in human NP cells treated with IL-1β or cotreated with IL-1β and UTI. . (b) The fluorescence strength of ROS (green) was quantified by Image J (). (c) IL-1β-induced differential levels of SOD and MDA were assessed by ELISA with UTI in a dose-dependent manner in human NP cells (). (d, e) Protein bands and quantification of protein levels of NOX4, NOX2, and SOD1 (). , , , .

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