Effect of Omega-3 or Omega-6 Dietary Supplementation on Testicular Steroidogenesis, Adipokine Network, Cytokines, and Oxidative Stress in Adult Male Rats

Table 1

Specific primers used for the analysis of different gene expressions.

Gene	GenBank accession no.	Primer sequences (5-3)	Orientation	Product size (bp)
STAR	NM_031558.3	CACAGTCATCACCCATGAGC	Forward	166
		AGCTCTGATGACACCGCTTT	Reverse
CYP17A1	NM_012753	CTCTGGGCACTGCATCAC	Forward	114
		CAAGTAACTCTGCGTGGGT	Reverse
CYP19	NM_017085	GCCTGTCGTGGACTTGGT	Forward	142
		GGTAAATTCATTGGGCTTGG	Reverse
3β-HSD	M38178	TGTGCCAGCCTTCATCTAC	Forward	145
		CTTCTCGGCCATCCTTTT	Reverse
17β-HSD	NM_054007	GACCGCCGATGAGTTTGT	Forward	140
		TTTGGGTGGTGCTGCTGT	Reverse
CYP11A1	J05156	CTTTGGTGCAGGTGGCTAG	Forward	115
		CGGAAGTGCGTGGTGTTT	Reverse
GAPDH	NM_017008.4	GTGCCAGCCTCGTCTCATAG	Forward	122
		CGTTGATGGCAACAATGTCCA	Reverse

¹Steroidogenic acute regulatory protein (StAR); 17α-hydroxylase/17, 20-lyase (CYP17A1); aromatase cytochrome P450 (CYP19); 3β-hydroxysteroid dehydrogenase (3β-HSD); 17β-hydroxysteroid dehydrogenase (17β-HSD); cholesterol side-chain cleavage enzyme (CYP11A1); glyceraldehyde 3-phosphate dehydrogenase (GAPDH).