Research Article

Loss of Function of von Hippel-Lindau Trigger Lipocalin 2-Dependent Inflammatory Responses in Cultured and Primary Renal Tubular Cells

Figure 1

Effect of VHL on ROS production in HK-2 cells. HK-2 cells were transfected with a vector expressing a scrambled shRNA (SC) or shRNA specific for VHL (shVHL1) (V1) (4) by electroporation with the Gemini X2 System and incubated for 48 hours. (a) After transfection, the intracellular ROS levels were determined using DCFDA. (b) The fluorescence intensity was detected using FACSCalibur analysis. All data were presented as the . as compared to the scrambled vector.
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