The Downregulation of ADAM17 Exerts Protective Effects against Cardiac Fibrosis by Regulating Endoplasmic Reticulum Stress and Mitophagy

Figure 3

ADAM17 regulates ER stress and mitophagy to promote the activation of mCFs. (a) Protein expressions of Smad2/3, p-Smad2/3, p-PERK, ATF4, p-IRE1, XBP1s, c-ATF6, and GRP78 in mCFs with NC or siRNA3 transfection in the presence or absence of TGF-β1 (). (b) The protein levels of the ER stress branch, the ATF6 and GRP78, after transfection of NC or siRNA3 in the presence or absence of TGF-β1 or TG (). (c, d) Wound healing and CCK-8 assays for migration and proliferation in mCFs transfected with NC or siRNA3 in the presence or absence of TGF-β1 or TG (). (e) Protein expression of PINK1 and Parkin with NC or siRNA3 transfection in the presence or absence of TGF-β1 in mCFs (). Data in (a–e) are expressed as . ns: not statistically significant; indicates , and indicates vs. the NC or NC+TGF-β1 groups.

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