Research Article

Antitumor Effects of Freeze-Dried Robusta Coffee (Coffea canephora) Extracts on Breast Cancer Cell Lines

Figure 3

Apoptosis induction by green coffee extract. MCF 10A, MDA-MB-231, and MCF7 cells were treated for 24 h with green coffee at 0, 500, or 1000 μg/mL. (a) Apoptosis flow cytometry analysis by Annexin V/propidium iodide staining was performed, and representative plots are presented. (b) Quantification of apoptosis induction (relative to control). Values are from three independent experiments () as determined by ANOVA, with Tukey’s multiple comparison posttest.
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