Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo

Figure 6

P62 mediated GSDMD-N degradation via an autophagy-lysosome pathway. (a) Immunoprecipitation for P62 was conducted to detect the integration of P62 and GSDMD-N. IgG was as a negative control. WCL: whole cell lysate. (b) Confocal images of GSDMD-N (green) and P62 (red) and fluorescence intensity results (right panel). (c) The mRNA level of GSDMD was analyzed by qRT-PCR. vs. the control group; ns, , no significant difference. (d) Overlap coefficient based on immunofluorescence images showed the colocalization relationship of GSDMD-N and P62. and vs. the control group. (e) Immunoprecipitation for P62 was conducted to detect the integration of P62 and GSDMD-N in CQ (20 μM, 24 h) or MG132 (10 μM, 24 h) treated NP cells under LPS stimulation. (f) Confocal images of GSDMD-N (green) and P62 (red) and fluorescence intensity measurement (right panel). (g) Overlap coefficient analyzed the colocalization of GSDMD-N and P62. vs. the CQ group. Data were presented as the , .

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