Kirenol Inhibits B[a]P-Induced Oxidative Stress and Apoptosis in Endothelial Cells via Modulation of the Nrf2 Signaling Pathway

Figure 3

B[a]P inhibits the PI3/AKT-mediated Nrf2 signaling. (a) Cells were incubated with different concentrations of B[a]P for 24 hours, with equal amounts of whole cell lysate being exposed to SDS-PAGE. Membranes probed with anti-pPI3K and anti-pAKT antibodies and PI3K and AKT levels were deemed internal control. (b) HUVECs cells were treated with B[a]P (5 μmol) for 0, 3, 6, 12, and 24 hours, and the membranes were probed with anti-pPI3K and anti-pAKT antibodies. In addition, PI3K and AKT levels were also regarded as internal control. (c) The treatment of cells with B[a]P (5 μmol) for different indicated periods. After preparing extracts of total protein, western blot assessments were undertaken and analyzed Nrf2, Ho-1, and NQO-1 activations. Data is shown as the of triplicate values (), whereas denotes a major discrepancy when compared to the control group; on the other hand, ^# represents noteworthy difference when compared to the B[a]P alone and KRL with the B[a]P treatment groups.

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