Programmed NP Cell Death Induced by Mitochondrial ROS in a One-Strike Loading Disc Degeneration Organ Culture Model

Figure 2

MS induced large-scale mitochondrial ROS generation and mitochondrial dysfunction. (a) Typical confocal images showing the viable cells (green) and ROS-positive cells (red) in the CON group and the MS group at 0 hours after MS. (b) Percentages of ROS-positive cells measured in the CON group and the MS group at different time points. (c) Rotenone and MitoQ pretreatment reduced the percentage of ROS-positive cells. (d) Typical confocal images of JC-1 staining showing the red fluorescence of JC-1 aggregates and the green signal of JC-1 monomers at 0 hours after MS. (e) Quantification of the mitochondrial membrane potential (ratio of red to green fluorescence) in the CON, MS, and MitoQ pretreatment groups. (f) The relative ratio of GSSG to total glutathione was measured at 12 hours after MS. (g, h) Quantification of SOD2 and CAT mRNA levels at 12 hours after MS. Scale bars, 50 μm and 5 μm (inset). The values are expressed as the . , , and .

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