High-Fat Diet-Induced Fatty Liver Is Associated with Immunosuppressive Response during Sepsis in Mice

Figure 2

High-fat diet septic mice are in a state of immune dysregulation. (a) The number of neutrophils labeled with CD11b and LY6G in the peritoneal lavage fluid (PLF) of mice was determined by flow cytometry. NC (), HFD (), NC+LPS (), and HFD+LPS (). (b) The number of macrophages labeled with CD11b and F4/80 in PLF of mice was determined by flow cytometry. NC (), HFD (), NC+LPS (), and HFD+LPS (). (c) Gene expressions of inflammatory cytokines and chemokines (NC , HFD , NC+LPS , and HFD+LPS ) analyzed by quantitative RT-PCR (relative to 18S). (d) The relative protein levels of TNF-α (NC , HFD , NC+LPS , and HFD+LPS ) and IL-6 (NC , HFD , NC+LPS , and HFD+LPS ) in PLF. by two-tailed Student’s -test.

(a)

(b)

(c)

(d)