Oxidative Medicine and Cellular Longevity / 2021 / Article / Fig 2

Research Article

Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy

Figure 2

Effects of graded mechanical compression on cell apoptosis, senescence, and ECM homeostasis of the human NP cells cultured in a bioreactor: (a) the early- and late-apoptosis rate of the NP cells that exerted graded compression loading was analyzed by flow cytometry. (b) Statistic analysis of the apoptosis rate of the NP cells that exerted graded compression loading. (c) Western blotting analysis of the expression level senescence-associated markers (P53, P21, and P16) and main ECM functional components (aggrecan and collagen type II) of the NP cells that exerted graded compression loading. (d) Statistic analysis of the western blots of the NP cells that exerted graded compression loading. . #.
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