Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy

Figure 4

TMT-based quantitative proteomics analyzed the differentially expressed proteins and pathways of the human NP cells that exerted graded mechanical compression loading: (a) the volcano plots revealed the DEPs of the NP cell-encapsulated hydrogels cultured under dynamical compression with 0% (control), 5% (low-compression loading), and 20% deformation (high-compression loading). (b) The Venn diagrams revealed the potential protective proteins (upregulated under low-compression loading but downregulated under high-compression loading) and adverse proteins (downregulated under low-compression loading but upregulated under high-compression loading) of the NP cells that suffered mechanical compression. (c) The heat map revealed part of the DEPs among the 0% (control), 5% (low-compression loading), and 20% deformation (high-compression loading) groups. (d) GO analysis of the DEPs between low-compression loading and high-compression loading groups revealed that the top enriched biological process was ATP biosynthetic process. (e) KEGG enrichment analysis of the DEPs between low-compression loading and high-compression loading groups revealed that the top enriched pathway was related to oxidative phosphorylation. and .

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