Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy

Figure 5

Effects of overloaded compression on biological behavior and MIF expression of the rat tail NP: (a) the close-up and X-ray views of the experimental rat tail IVD under the condition of overloaded compression. (b) The MRI image of the experimental rat tail IVD under the different duration of overloaded compression. (c) Statistic analysis of the MRI Pfirrmann grade changes of the experimental rat tail IVD under the different duration of overloaded compression. (d) The HE staining showed the histomorphological changes of the experimental rat tail IVD under the different duration of overloaded compression. (e) Statistic analysis of the Masuda degenerative score changes of the experimental rat tail IVD under the different duration of overloaded compression. (f) The Alcian blue staining showed the glycosaminoglycan content of the experimental rat tail IVD under the different duration of overloaded compression. (g) Statistic analysis of Alcian blue staining AOD value of the experimental rat tail IVD under the different duration of overloaded compression. (h) MIF IHC staining of the experimental rat tail IVD under the different duration of overloaded compression. (i) Statistic analysis of MIF IHC staining AOD value of the experimental rat tail IVD under the different duration of overloaded compression. . ^#.

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