Oxidative Medicine and Cellular Longevity / 2021 / Article / Fig 6

Research Article

Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy

Figure 6

MIF-KO exacerbated oxidative stress-induced senescence of the NP cell that suffered overloaded compression: (a) after MIF-KO and high-compression loading treatment, the cell senescence degree of the NP cells was analyzed by SA-β-Gal staining (100x). (b) Statistic analysis of the SA-β-Gal-positive rate of the NP cells. (c) After MIF-KO and high-compression loading treatment, the ROS content of the NP cells was measured by flow cytometry following DCFH-DA staining. (d) Statistic analysis of the ROS content of the NP cells. (e) Western blotting analysis of the expression levels of MIF, oxidative stress biomarker NT, and senescence-associated markers (P53, P21, and P16) of the NP cells. (f) Statistic analysis of the western blots of the biomarkers. . #. .
(a)
(b)
(c)
(d)
(e)
(f)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.