Deficiency of MIF Accentuates Overloaded Compression-Induced Nucleus Pulposus Cell Oxidative Damage via Depressing Mitophagy
MIF-KO accentuates oxidative stress-induced senescence and mitochondrial dysfunction of the NP cell: (a) western blotting analysis of the expression levels of the mitophagy-related markers (PINK1, Parkin, LC3II/I, and P62) of the NP cells. (b) Statistic analysis of the western blots of the mitophagy-related biomarkers. (c) The mitochondrial membrane potential was observed and measured by fluorescence microscopy after JC-1 fluorescent molecular probe staining. (d) Statistic analysis of the JC-1 green/red fluorescence intensity ratio. .#.
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