HIF-1α-Mediated miR-623 Regulates Apoptosis and Inflammatory Responses of Nucleus Pulposus Induced by Oxidative Stress via Targeting TXNIP

Figure 5

miR-623 attenuates TBHP-induced apoptosis and inflammation via TXNIP. (a) Western blot analysis of TXNIP expression in NP cells induced by TBHP with or without miR-623 mimic transfection. (b) Western blot analyses of TXNIP, BAX, BCL2, cleaved caspase-3, collagen II, iNOS, MMP13, and IL-1β in NP cells induced by TBHP with or without TXNIP overexpression and/or miR-623 mimic transfection. (c, d) Quantification analysis of IL-1β and IL-6 mRNA expression in NP cells induced by TBHP with or without TXNIP overexpression and/or miR-623 mimic transfection. (e) Quantification analysis of the secretion of IL-6 in culture medium of NP cells induced by TBHP with or without TXNIP overexpression and/or miR-623 mimic transfection. . values were analyzed by two-way ANOVA.

(a)

(b)

(c)

(d)

(e)