YAP-Dependent Induction of CD47-Enriched Extracellular Vesicles Inhibits Dendritic Cell Activation and Ameliorates Hepatic Ischemia-Reperfusion Injury

Figure 1

Silencing of YAP exacerbates liver injury and decreases sEVs’ concentration during IRI. (a) Western blot analysis of hepatic YAP protein levels of IRI mice at different time points; (b) the mRNA levels of hepatic YAP from IRI mouse liver were detected by qPCR at different time points. (c) H&E staining of ischemic livers in WT and YAP-HKO mice showed that the injury increased with reperfusion time and reached the peak at 24 h after reperfusion. Scale bar, 1 mm. (d) Serum ALT levels of YAP-HKO mice were significantly higher than WT mice with I/R at 24 h ( U/L vs.  U/L, ). (e) Suzuki’s histological score of YAP-HKO mice was higher than WT mice with I/R at 24 h (score: vs. score: , ). (f) The concentrations of sEVs from WT and YAP-HKO groups at different time points after IRI, as measured by CD81 exosome ELISA kit. per group. , ns: , -test.

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