Research Article

YAP-Dependent Induction of CD47-Enriched Extracellular Vesicles Inhibits Dendritic Cell Activation and Ameliorates Hepatic Ischemia-Reperfusion Injury

Figure 5

EVs released from hepatocytes inhibit DC activation in a YAP-dependent manner. (a) Primary BMDCs were treated by a conditioned medium from WT or YAP-HKO PMH and were stimulated with LPS. BMDCs were then cocultured with CD4+ and CD8+ T cells isolated from BALB/c mice at the ratio of 1 : 10. The proliferation of CD4+ and CD8+ T cells was measured by CFSE-MLR. (b) The extent of T cell proliferation in (a) was analyzed. (c) BMDCs were treated with a conditioned medium or EVs from WT or YAP-HKO PMH culture supernatants, and then, the IL-12 p40 level in the culture medium was determined by ELISA. ; ; . .
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