RIPK3-Mediated Necroptosis in Diabetic Cardiomyopathy Requires CaMKII Activation

Figure 4

RIPK3 deficiency decreases RIPK1 expression, MLKL phosphorylation, and CaMKII activity and improves myocardial mitochondrial ultrastructure in DCM. (a) RIPK1 expression in cardiomyocytes was quantified by western blot. GAPDH was used as a loading control. (b) MLKL phosphorylation (p-MLKL) and total MLKL were quantified by western blot. GAPDH was used as a loading control. (c, d) Expression of CaMKII oxidation (ox-CaMKII), CaMKII phosphorylation (p-CaMKII), and total CaMKII was quantified by western blot. GAPDH was used as a loading control. (e) Myocardial mitochondrial ultrastructure was examined with a transmission electron microscope. Scale bars: 2 μm (upper) and 0.5 μm (lower), respectively. , significantly from WT; ^## and #, significantly from WT-DCM. .

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