HO-1/CO Maintains Intestinal Barrier Integrity through NF-κB/MLCK Pathway in Intestinal HO-1^-/- Mice

Figure 4

HO-1 and MLCK in intestinal epithelial cells mediate barrier loss. (a, b) Representative protein bands and quantification analyses of a Western blot for long MLCK, p-MLC-2/MLC-2, ZO-1, and occludin in Caco-2 cells transfected with FUGW-HO-1 plasmid after pretreatment of ML-7 with or without TNF-α stimulation. (c, d) Representative protein bands and quantification analyses of a Western blot for long MLCK, p-MLC-2/MLC-2, ZO-1, and occludin in Caco-2 cells transfected with pLKO.1-sh-HO-1 plasmid after pretreatment of ML-7 with or without TNF-α stimulation. All data presented were representative of three or more independent experiments, each with similar results. and .

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