Antioxidant and Anti-Inflammatory Profiles of Spent Coffee Ground Extracts for the Treatment of Neurodegeneration

Figure 6

Protein level of HO1 in SH-SY5Y cells treated with the extracts and exposed to H₂O₂. Cells were treated with E1, E2, E3, and E4 (50 μg mL⁻¹) and after 24 h exposed to H₂O₂ 700 μM for 1 h. Immunoblotting was performed using anti-HO1. Data are expressed as fold over CTRL and normalized by β-actin. Each bar represents of three independent experiments. Data were analyzed with a one-way ANOVA followed by the Bonferroni’s test. ° vs. CTRL; vs. H₂O₂.