Research Article

Molecular Basis for the Interactions of Human Thioredoxins with Their Respective Reductases

Figure 1

Molecular interaction surfaces of the human Trx1-TrxR1 and E. coli Trx1-TrxR complexes. Cartoon representation of the secondary structures of (a) human Trx1-TrxR1 complex PDB: 3qfa and (d) E. coli Trx1-TrxR complex PDB: 1f6m. The complex structure was opened by rotating both Trx and TrxR structures by 90° forward and backward, respectively. The contact patches with direct molecular contacts were highlighted in yellow using UCSF chimera (b, e). The isosurfaces of the electrostatic potential were depicted at ±1 in blue (positive) and red (negative), respectively. The active site cysteinyl residues and interaction surfaces in the immediate contact area in both proteins were encircled in white lines (c, f).
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