Fisetin Alleviates Neointimal Hyperplasia via PPARγ/PON2 Antioxidative Pathway in SHR Rat Artery Injury Model

Figure 7

Fisetin inhibited neointimal hyperplasia after balloon injury of carotid artery in SHR rats. Neointimal hyperplasia of carotid artery was induced after balloon injury in SHR rats. Fisetin (3 mg/kg) or vehicle was intraperitoneally injected daily for two weeks. Then, carotid artery was harvested for neointima examination. (a) Representative images of hematoxylin-eosin (HE) cross-sections in different groups were shown at 10x and 40x magnifications. (b) The ratio of intima (I) to media (M) was compared between vehicle and fisetin groups. (c) Representative immunofluorescence images of PCNA (green) and DAPI (blue) ( μm). The percentage of PCNA-positive cells counted under 40x magnification was compared ( vs. vehicle, /group). The level of (d) MDA and (e) T-AOC (total cellular antioxidant capacity) activity in carotid artery were measured. (f) The effect of fisetin on PON2 protein expression in carotid artery determined by Western blotting ( vs. sham and ^# vs. vehicle, /group).

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