Long Noncoding RNA ANPODRT Overexpression Protects Nucleus Pulposus Cells from Oxidative Stress and Apoptosis by Activating Keap1-Nrf2 Signaling

Figure 4

ANPODRT activates Nrf2 signaling in human NP cells. (a–d) The protein expression of Nrf2 in human NP cells transfected with oe-ANPODRT, si-ANPODRT, or negative control was measured by Western blotting. (e, f) The mRNA expression of Nrf2 in human NP cells transfected with oe-ANPODRT, si-ANPODRT, or negative control was measured by qRT-PCR. (g–j) The protein expression of nucleus Nrf2 and cytoplasm Nrf2 was measured by Western blotting. (k–n) The mRNA expression of HO1 and NQO1 in human NP cells transfected with oe-ANPODRT, si-ANPODRT, or negative control was measured by qRT-PCR. Data are represented as the . , , and versus si-NC/oe-NC group; N.S.: not significant.

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