Long Noncoding RNA ANPODRT Overexpression Protects Nucleus Pulposus Cells from Oxidative Stress and Apoptosis by Activating Keap1-Nrf2 Signaling

Figure 5

ANPODRT disrupts the Keap1-Nrf2 complex formation in human NP cells. (a–d) Human NP cells were transfected with oe-ANPODRT, si-ANPODRT, or negative control followed by MG132 treatment. The protein level of Nrf2 in human NP cells was measured. (e–h) The protein level of Keap1 and Nrf2 in human NP cells transfected using oe-ANPODRT/si-ANPODRT and si-Keap1. (i, j) The mRNA level of HO1 and NQO1 in human NP cells transfected using oe-ANPODRT/si-ANPODRT and si-Keap1. (k–n) The protein level of Keap1 in human NP cells transfected with oe-ANPODRT, si-ANPODRT, or negative control. (o, p) After oe-ANPODRT/si-ANPODRT transfection, the interaction between Keap1 and Nrf2 was determined by Co-IP assay. Data are represented as the . versus control group; N.S.: not significant.

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