The Secretome Deregulations in a Rat Model of Endotoxemic Shock

Figure 2

Flow chart of inclusion and exclusion procedure used to obtain a list of deregulated protein. The animals were divided into 4 groups (CT50, LPS50, CT90, and LPS90). Physiological parameters made it possible to include 24 rats in the study. For each rat, 2 time points were studied (T0 and T50 or T0 and T90) and were analyzed by mass spectrometry ( samples), and proteomic results were analyzed by RStudio software. Proteins with a maximum of 2 missing data (NA) per sample and a coverage (Cov) greater than 15% were selected. A total of 140 proteins in group T50 and 132 proteins in group T90 were included. The deregulated proteins (DEP) present in the controls were excluded from the study.